Antibody Radiolabeling Screening

Antibody Radiolabeling Screening

One way the immune system attacks foreign substances in the body is by making large numbers of antibodies. An antibody is a protein that sticks to a specific protein called an antigen. Antibodies circulate throughout the body until they find and attach to the antigen. Once attached, they can recruit other parts of the immune system to destroy the cells containing the antigen.

Researchers can design antibodies that specifically target a certain antigen, such as one found on cancer cells. They can then make many copies of that antibody in the lab. These are known as monoclonal antibodies (mAbs).

Over the past couple of decades, the US Food and Drug Administration (FDA) has approved more than a dozen mAbs to treat certain cancers. As researchers have found more antigens linked to cancer, they have been able to make mAbs against more and more cancers.

METIS Labs can label your antibodies with radioiodine using a simple procedure. The labeled primary antibody can then be used to directly measure both antibody affinity (Kd) and the density of antigen sites (sites/cell) in live cells. Radioactive assays often have higher sensitivity, robustness and quantification when compared with comparable fluorescent-based assays.

Other Services In Antibody Radiolabeling Screening

Antibody Radiolabeling

Stock Antibody and Na[125I]Iodide are mixed with IodoGenĀ®. After a set amount of time the mixture is transferred to a fresh vial and the reaction is quenched.

Antibody (Ab) Saturation Assay (Sites/Cell & Kd)

Multiple concentrations (up to twelve) of the [125I]-Ab are incubated in quadruplicate with a desired cell line containing the target of interest.

Antibody (Ab) Competition Binding Assay (Ic50 And Ki)

Live cells containing the target of interest are incubated together with a [125I]-Ab at a single concentration along with 10 - 12 concentrations of unlabeled Ab in triplicate (or quadruplicate) replicates at each concentration.

Antibody (Ab) Homologous Competition (Bmax & Kd)

A homologous competition is a concentration response curve with an unlabeled compound that is identical to the radioligand being used.

Case Studies