Functional Binding Assay 96-Well Plate

Functional Binding Assay 96-Well Plate

[35S]-GTPγS binding, also known as a GTP exchange assay, is a powerful method used to measure the activation of G protein-coupled receptors (GPCRs) by a ligand.

[35S]-GTPγS binding can be used to:

Determine if a ligand can activate a GPCR of interest

Single concentration screening or dose-response to determine pharmacological values of potency (EC50) & efficacy (EMAX)

Determine full, partial, or inverse agonist and antagonist activity

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