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Resources / Dilution Calculator

Dilution Calculator

Dilution Calculator

Dilution refers to make a lower concentration solution from higher concentrations. Solutions usually are stored in a higher concentration, for convenience of use and avoiding contamination.

The METIS Labs dilution calculator is a useful tool which allows you to calculate the

  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Prepare stock solutions
  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Dilutions from stock solutions
  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Serial dilutions of stock solutions
Calculate the dilution required to prepare a stock solution
Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
C1
V1
C2
V2
The dilution calculator is based on the following equation: Concentration(start) x Volume(start) = Concentration(final) x Volume(final)

An example of a dilution calculation

What volume of a given 10 mM stock solution is required to make 20 ml of a 50 μM solution?

Using the equation C1 V1 = C2 V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:

1. Enter 10 into the Concentration (start) box and select the correct unit (millimolar)

2. Enter 50 into the Concentration (final) box and select the correct unit (micromolar)

3. Enter 20 into the Volume (final) box and select the correct unit (milliliter)

4. Press calculate

5. The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

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