Saturation Binding Assays (BMAX/KD)

Saturation Assay (BMAX & KD)

Multiple concentrations (up to twelve) of the radioligand are incubated in triplicate or quadruplicate with either cell, or tissue homogenate containing the target receptor of interest.

Filter-based Separation

Scintillation Proximity Assay (SPA)

The filters or SPA plate are counted for radioactivity to determine the level of receptor-bound radioligand and the results are plotted to obtain the IC50 and Ki values for the drug-receptor interaction.

Example Saturation Binding Graph

The filters or SPA plate are counted using liquid scintillation counting to determine the level of receptor-bound radioligand and the results plotted to obtain the receptor density (BMAX) and if desired the Kd for the receptor-radioligand integration.

How We Work

Case Studies