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services / Antibody Radiolabeling & Screening / Antibody (Ab) Competition Binding Assay (IC50 and Ki)

Antibody (Ab) Competition Binding Assay (IC50 and Ki)

Antibody (Ab) Competition Binding Assay (IC50 and Ki)

Live cells containing the target of interest are incubated together with a [125I]-Ab at a single concentration along with 10 – 12 concentrations of unlabeled Ab in triplicate (or quadruplicate) replicates at each concentration.

After equilibrium has been reached, receptor-bound radioligand is separated from non-bound radioligand using either.

  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Filter-based Separation
  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Scintillation Proximity Assay (SPA)

The filters or SPA plates are counted for radioactivity to determine the level of target-bound radioligand and the results are plotted to obtain the IC50 and Ki values for the Ab-target interaction.

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Compound Characterization

Compound Characterization

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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Compound Characterization 1

Compound Characterization 1

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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