888-295-7550 info@metislabs.com
services / Antibody Radiolabeling & Screening / Antibody (Ab) Saturation Assay (Sites/cell & Kd)

Antibody (Ab) Saturation Assay (Sites/cell & Kd)

Antibody (Ab) Saturation Assay (Sites/cell & Kd)

Multiple concentrations (up to twelve) of the [125I]-Ab are incubated in quadruplicate with a desired cell line containing the target of interest.

After equilibrium has been reached, receptor-bound [125I]-Ab is separated from non-bound radioligand using either:

  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Filter-based Separation
  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Scintillation Proximity Assay (SPA)

The filters or SPA plate are counted using liquid scintillation counting, or Gamma Counting, to determine the level of receptor-bound radioligand and the results plotted to obtain sites/cell and if desired the Kd for the target-radioligand integration.

How We Work

Brainstorming

1

Research

2

Report

3

Case Studies

Compound Characterization

Compound Characterization

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

View More
Compound Characterization 1

Compound Characterization 1

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

View More

Call Us On

888-295-7550