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In Vitro Receptor Autoradiography

In Vitro Receptor Autoradiography

With the use of radiolabeled ligands, in vitro Autoradiography studies can be used to localize receptors (receptor distribution) and characterize drug binding sites in tissue sections.

For these studies, cryostat-cut sections are incubated in vitro in a receptor specific buffer containing the radiolabeled drug. Once equilibrium binding conditions are achieved, the sections are washed, dried and imaged for radioactivity using either phosphor-imaging or beta imaging.

Autoradiogram of Human, Monkey and Dog Cerebellum

Autoradiogram of Monkey Tissue

Autoradiogram of Rodent Tissue

Bidning of NMDA Receptors using [3H] MK801

Bidning of NMDA Receptors using [3H] MK801

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Compound Characterization

Compound Characterization

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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Compound Characterization 1

Compound Characterization 1

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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