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Bolton-Hunter Reagent Radioiodination

Bolton-Hunter Reagent Radioiodination

Bolton-Hunter reagent is the N-hydroxysuccinimide ester of iodinated p-hydroxyphenylpropionic acid. Bolton-Hunter method labels terminal amino groups with a radioiodinated phenolic residue, effectively introducing radioactive iodine into proteins and peptides. Since it is a non-oxidative technique, it is “less harsh” to proteins than alternative methods in turn retaining a high degree of binding activity to receptors.

The mono-iodinated form is generally recommended for most Bolton-Hunter iodinations. The di-iodinated form is the same as the mono-iodinated with the difference of doubling the specific activity (4400 Ci/mmol) for each molecule of reagent. This is used when the extra sensitivity is important relative to decrease in stability.

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Compound Characterization

Compound Characterization

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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Compound Characterization 1

Compound Characterization 1

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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