Radioiodine ([125I]) is widely used in the preparation of tracers for sensitive immunoassays and other procedures for the detection, localisation and quantification of substances in biological samples, for example, receptor binding assays or autoradiography.
[125I] can be introduced into proteins/peptides either indirectly by conjugating to a ready labelled compound such as [125I]-Bolton & Hunter reagent or directly using a soluble oxidizing agent such as chloramine-T. Indirect or conjugation methods are milder for proteins by avoiding exposure to potentially denaturing conditions during the radiolabelling procedure. Following radiolabelling, the tracer is carefully purified to remove unreacted [125I] and other reaction by-products by specialized methods.
METIS Labs Custom Radioiodination Services offers a wide range of labeling techniques to best maintain the biological activity of your molecule to advance your peptide, protein or antibody development pipeline.