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services / Radioligand Binding Assays / Saturation Binding Assays (BMAX/KD)

Saturation Binding Assays (BMAX/KD)

Saturation Assay (BMAX & KD)

Multiple concentrations (up to twelve) of the radioligand are incubated in triplicate or quadruplicate with either cell, or tissue homogenate containing the target receptor of interest.

  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Filter-based Separation
  • Created by potrace 1.10, written by Peter Selinger 2001-2011 Scintillation Proximity Assay (SPA)

The filters or SPA plate are counted for radioactivity to determine the level of receptor-bound radioligand and the results are plotted to obtain the IC50 and Ki values for the drug-receptor interaction.

Radioligand binding assays 02

Example Saturation Binding Graph

The filters or SPA plate are counted using liquid scintillation counting to determine the level of receptor-bound radioligand and the results plotted to obtain the receptor density (BMAX) and if desired the Kd for the receptor-radioligand integration.

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Compound Characterization

Compound Characterization

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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Compound Characterization 1

Compound Characterization 1

Compound Characterization Study Goal Determine ex vivo receptor occupancy of CMPDx at Orexin-1 (OX1) receptors using radiolabeled Orexin antagonist Created by potrace 1.10, written by Peter Selinger 2

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